Figure 5

PER 2 expression increases P53 but decreases cyclin D1 expression in MCF-7 cells. (A) MCF-7 cells were transiently transfected with and empty vector (pcDNA 3.1) [U] or vector expressing PER 2 [T]. After 48 hours, total cell extracts were prepared and subjected to Western blot analysis using an antibodies directed against the P53 and PER 2 proteins. (B) Densitometric analysis of P53 protein expression. * p < 0.05. (C) Western blot analysis using an antibodies directed against the cyclin D1(cD1) and PER 2 proteins. (D) Densitometric analysis of cD1 protein expression. * p < 0.05.