Figure 2

Individual release patterns of AVP from in vitro SCN slice cultures. Slice cultures were made from wildtype (upper panels) and Per1 ^m/m Per2 ^m/mdouble-mutant mice (lower panels). Release pattern is shown as a function of external time, extrapolated from time of sacrifice. Right panels show release patterns of mice sacrificed during LD, and left panels show release patterns of mice sacrificed during the first day of DD. All cultures showed significant circadian rhythmicity in AVP release pattern with periods between 18–32 h, between ExT 10–44 h (except one culture from WT mice under LD). Different line types indicate SCN-AVP release of an individual culture. Black/grey bars indicate projected night and day, respectively.