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Table 2 Characteristics of the diurnal rhythms of hepatic calcium-handling proteins from rats under free access meal and restricted schedule determined by COSINOR test

From: Diurnal and nutritional adjustments of intracellular Ca2+ release channels and Ca2+ ATPases associated with restricted feeding schedules in the rat liver

  

Acrophase ZT (h)

Amplitude

MESOR

Rhythmicity ( P < 0.05 )

  

AL

RF

AL

RF

AL

RF

AL

RF

Ca 2+ releasing proteins

IP 3 R (ER Binding assay)

12:20 + 00:21

04:02 + 00:20*

1.3 + 0.22

0.6 + 0.10*

2.6 + 0.44

1.5 + 0.21*

0.01

0.05

IP 3 R1 (WB)

12:05 + 00:35

05:37 + 00:47*

0.28 + 0.04

0.16 + 0.03*

0.56 + 0.08

0.37 + 0.06*

0.03

0.04

IP 3 R (PM Binding assay)

09:57 + 00:48

04:22 + 00:28*

42.8 + 10.3

36.3 + 6.1

82.9 + 21.7

61.9 + 12.3*

0.02

0.05

IP 3 R2 (WB)

11:26 + 00:29

04:03 + 00:31*

0.29 + 0.034

0.25 + 0.036

0.54 + 0.07

0.31 + 0.07*

0.02

0.03

RyR (Binding assay)

17:43 + 00:41

17:10 + 00:32

3.2 + 0.46

4.3 + 0.78

9.7 + 0.9

10.2 + 1.5

0.03

0.05

RyR (WB)

17:50 + 00:50

17:30 + 00:58

0.29 + 0.033

0.22 + 0.052

0.59 + 0.06

0.62 + 0.10

0.03

0.3NS

Ca 2+ extruding proteins

SERCA (Activity)

18:35 + 00:31

03:51 + 00:39*

5.36 + 0.9

20.9 + 2.4*

23.2 + 2.1

47.4 + 5.8*

0.2NS

0.004

SERCA2 (WB)

16:48 + 00:29

02:55 + 00:24*

0.24 + 0.02

0.34 + 0.03*

0.49 + 0.04

0.66 + 0.07*

0.4NS

0.02

PMCA (Activity)

21:48 + 01:09

23:44 + 01:07

6.2 + 1.2.

2.7 + 0.8*

19.8 + 2.3

21.6 + 1.4

0.2NS

0.5NS

PMCA1 (WB)

01:35 + 0:59

09:15 + 00:45*

0.1 + 0.02

0.2 + 0.03

0.8 + 0.04

0.67 + 0.06

0.2NS

0.02

PMCA4 (WB)

12:48 + 00:32

17:37 + 00:24*

0.24 + 0.04

0.3 + 0.05

0.66 + 0.08

0.53 + 1.0

0.06NS

0.04

  1. The Acrophase, amplitude, and MESOR as well as the rhythmicity significance were determined as described in the methods section. Values were determined from the diurnal profiles of the parameters mentioned. The units corresponding to amplitude and MESOR are: fmoles/mg for IP3R and RyR binding assays; NADH/min/mg of protein for SERCA and PMCA activity; and normalized units for IP3R1-2, RyR and PMCA1-4 Western Blot (WB). * (p < 0.05) significant between AL vs RF (Student´s t-test). Not significant 24 h rhythmicity was designed as NS.