Acrophase ZT (h) | Amplitude | MESOR | Rhythmicity ( P < 0.05 ) | ||||||
---|---|---|---|---|---|---|---|---|---|
AL | RF | AL | RF | AL | RF | AL | RF | ||
Ca 2+ releasing proteins | IP 3 R (ER Binding assay) | 12:20 + 00:21 | 04:02 + 00:20* | 1.3 + 0.22 | 0.6 + 0.10* | 2.6 + 0.44 | 1.5 + 0.21* | 0.01 | 0.05 |
IP 3 R1 (WB) | 12:05 + 00:35 | 05:37 + 00:47* | 0.28 + 0.04 | 0.16 + 0.03* | 0.56 + 0.08 | 0.37 + 0.06* | 0.03 | 0.04 | |
IP 3 R (PM Binding assay) | 09:57 + 00:48 | 04:22 + 00:28* | 42.8 + 10.3 | 36.3 + 6.1 | 82.9 + 21.7 | 61.9 + 12.3* | 0.02 | 0.05 | |
IP 3 R2 (WB) | 11:26 + 00:29 | 04:03 + 00:31* | 0.29 + 0.034 | 0.25 + 0.036 | 0.54 + 0.07 | 0.31 + 0.07* | 0.02 | 0.03 | |
RyR (Binding assay) | 17:43 + 00:41 | 17:10 + 00:32 | 3.2 + 0.46 | 4.3 + 0.78 | 9.7 + 0.9 | 10.2 + 1.5 | 0.03 | 0.05 | |
RyR (WB) | 17:50 + 00:50 | 17:30 + 00:58 | 0.29 + 0.033 | 0.22 + 0.052 | 0.59 + 0.06 | 0.62 + 0.10 | 0.03 | 0.3NS | |
Ca 2+ extruding proteins | SERCA (Activity) | 18:35 + 00:31 | 03:51 + 00:39* | 5.36 + 0.9 | 20.9 + 2.4* | 23.2 + 2.1 | 47.4 + 5.8* | 0.2NS | 0.004 |
SERCA2 (WB) | 16:48 + 00:29 | 02:55 + 00:24* | 0.24 + 0.02 | 0.34 + 0.03* | 0.49 + 0.04 | 0.66 + 0.07* | 0.4NS | 0.02 | |
PMCA (Activity) | 21:48 + 01:09 | 23:44 + 01:07 | 6.2 + 1.2. | 2.7 + 0.8* | 19.8 + 2.3 | 21.6 + 1.4 | 0.2NS | 0.5NS | |
PMCA1 (WB) | 01:35 + 0:59 | 09:15 + 00:45* | 0.1 + 0.02 | 0.2 + 0.03 | 0.8 + 0.04 | 0.67 + 0.06 | 0.2NS | 0.02 | |
PMCA4 (WB) | 12:48 + 00:32 | 17:37 + 00:24* | 0.24 + 0.04 | 0.3 + 0.05 | 0.66 + 0.08 | 0.53 + 1.0 | 0.06NS | 0.04 |