Figure 2

Magel2 interacts with Bmal1 and Per2. A) HEK293 cells were transfected with expression plasmids encoding HA-Bmal1, Xpress-Magel2, or both constructs. Five percent of the volume of cell lysate used for immunoprecipitation was immunoblotted to confirm the presence of HA-Bmal1 and Xpress-Magel2 protein in the appropriate input samples (top). The protein complexes were immunoprecipitated with anti-HA or anti-Xpress antibodies, and the immunoprecipitates detected by immunoblotting with antibodies directed against the reciprocal epitope tag (bottom). B) HEK293 cells were transfected with expression plasmids encoding V5-Per2, Xpress-Magel2, or both constructs. Five percent of the volume of cell lysate used for immunoprecipitation was immunoblotted to confirm the presence of Xpress-Magel2 and V5-Per2 protein in the appropriate input samples (top). The protein complexes were immunoprecipitated with anti-V5 or anti-Xpress antibodies, and the immunoprecipitates detected by immunoblotting with antibodies directed against the reciprocal epitope tag (bottom).