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Figure 3 | Journal of Circadian Rhythms

Figure 3

From: Magel2, a Prader-Willi syndrome candidate gene, modulates the activities of circadian rhythm proteins in cultured cells

Figure 3

Subcellular localization of circadian proteins in the presence of Magel2. pXpressMagel2 and plasmids encoding other epitope-tagged circadian proteins (A, Clock, B, Bmal1, C, Per2, and D, Cry1) were detected in co-transfected NIH3T3 cells by immunofluorescence. Plots of fluorescence intensity were generated to assess overlap between the immunofluorescence signals from each protein within transfected cells, which were also stained with Hoechst 33342 to label the nucleus.

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