Figure 1

TIM protein levels and locomotor activity rhythm in flies receiving heat shock under DD conditions. D. ananassae TIM protein was induced by heat shock at PZT 10.5–11.0 (A) and at PZT 22.5–23.0 (B) in w, tim ⁰¹, hs-D. ananassae tim flies. Heat shock at 37°C for 30 minutes every day rapidly induced TIM. The dotted lines depict data from non-heat-shocked control flies. (C) and (D) are representative double-plot actgrams of locomotor activitys of transgenic flies that were heat shocked at PZT 10.5–11.0 (C) and PZT 22.5–23.0 (D), respectively. Dark and shaded bars show subjective night and day under DD conditions. Heat shock was applied for 30 minutes at 37°C every day. Arrowheads indicate start point of heat shock.