Figure 2

Mating activities of flies at different times of day. Error bars indicate SEM. Males and females were allowed to mate for 20 min. In each experiment, the male flies were crossed with female flies of the same genotype. (A) Mating activity rhythm of D. melanogaster Canton-S females crossed with D. melanogaster Canton-S males and in D. ananassae HW females crossed with D. ananassae HW males. (B) Mating activities in transgenic w, tim ⁰¹, hs-D. ananassae tim flies that were heat shocked every day at PZT 10.5–11.0. (C) Mating activities in transgenic w, tim ⁰¹, hs-D. ananassae tim flies that were heat shocked every day at PZT 22.5–23.0. (D) Mating activity rhythm of heat shocked D. melanogaster wild-type Canton-S female flies crossed with Canton-S males. Both males and females were applied heat shock at PZT 10.5–11.0.