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Figure 1 | Journal of Circadian Rhythms

Figure 1

From: Glycogen synthase kinase 3, circadian rhythms, and bipolar disorder: a molecular link in the therapeutic action of lithium

Figure 1

Circadian oscillation profiles of clock genes in wild-type and GSK3β -/- MEFs. A, wild type and B, GSK3β -/- cells were synchronized, harvested, processed, and the gene products were amplified as described in the Materials and Methods. The resulting transcriptional profiles of murine GAPDH, mPer2, mCry1, RevErbα, and Bmal1 were analyzed by reverse-transcription PCR. The subjective time points (TP) of peak expression are designated in white above the corresponding bands for each transcript examined. Panel C is a graphical depiction of mPer2 transcriptional oscillation based on relative values derived from densitometric measurements of PCR-amplified DNA bands in panels A and B expressed as percentages of the highest recorded value in each respective data set.

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Journal of Circadian Rhythms

ISSN: 1740-3391