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Figure 2 | Journal of Circadian Rhythms

Figure 2

From: Glycogen synthase kinase 3, circadian rhythms, and bipolar disorder: a molecular link in the therapeutic action of lithium

Figure 2

Analysis of GSK3α knockdown efficacy in a GSK3β nullizygous background. Puromycin-resistant, GSK3α-knockdown MEF lines were generated from GSK3β -/- MEFs as described in the Materials and Methods. A total of 13 individual clones (A1.3-A1.15) were subsequently isolated, expanded, and tested by Western blotting to determine the level of GSK3α knockdown, as well as any concomitant increase in cytosolic levels of β-Catenin protein. GAPDH levels were used as a loading control.

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Journal of Circadian Rhythms

ISSN: 1740-3391