Skip to main content
Figure 3 | Journal of Circadian Rhythms

Figure 3

From: Glycogen synthase kinase 3, circadian rhythms, and bipolar disorder: a molecular link in the therapeutic action of lithium

Figure 3

Circadian oscillation profiles of mPer2 following pharmacological or genetic GSK3 inhibition. The transcriptional profile mPer2 (A) was analyzed by reverse-transcription PCR in: wild-type, GSK3β -/-/GSK3α RNAi (clones A1.4 and A1.6); as well as 20 mM Lithium or 25 μM kenpaullone treatment in a wild-type background. The subjective time points of peak expression are designated in white above the corresponding bands for each transcript examined. The time intervals where these effects are most visible (TP0-4, TP24-32, and TP44-52) are isolated in white boxes. The effects of genetic (B) and pharmacological (C) interference of GSK3 activity on mPer2 transcriptional oscillation are graphically depicted based on relative values derived from densitometric measurements of PCR-amplified DNA bands in panel A expressed as percentages of the highest recorded value in each respective data set.

Back to article page

Journal of Circadian Rhythms

ISSN: 1740-3391