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Figure 5 | Journal of Circadian Rhythms

Figure 5

From: Glycogen synthase kinase 3, circadian rhythms, and bipolar disorder: a molecular link in the therapeutic action of lithium

Figure 5

Circadian oscillation profile of mPer2 in wildtype and GSK3β (-/-) ; GSK3α (flox/-) MEFs. The transcriptional profile of mPer2 and GAPDH were analyzed by reverse-transcription PCR in the A6(wt) and c2.1(3/4 DKO) cell lines, as depicted in panels A and B, respectively. Protein samples harvested from whole-cell lysates in parallel to RNA samples harvested for transcriptional analysis at corresponding time points were analyzed by SDS-PAGE electrophoresis. Western blot analysis of these protein samples for total-GSK3 and GAPDH is depicted in Panel C for TPs 0, 4, 12, 24, 30, and 36. Panels D and E are graphical depictions of relative levels of mPer2 expression in A6 and c2.1 based on three separately harvested A6 RNA sample sets and six c2.1 RNA sample sets. Relative values derived from densitometric measurements of PCR-amplified DNA bands are expressed as percentage values of mPer2 at TP1.

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Journal of Circadian Rhythms

ISSN: 1740-3391