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Figure 3 | Journal of Circadian Rhythms

Figure 3

From: Standards of evidence in chronobiology: critical review of a report that restoration of Bmal1 expression in the dorsomedial hypothalamus is sufficient to restore circadian food anticipatory rhythms in Bmal1-/- mice

Figure 3

Actogram-style plots and corresponding average waveforms of body temperature in food restricted mice (modified, with permission, from Fuller et al [12]© (2008) AAAS http://www.sciencemag.org , Figure S3). For clarity, we have placed the waveform figures under the corresponding actogram-style figures. According to the text, these 2 waveforms were derived from days 10–14 of restricted feeding. We have aligned the waveforms and corresponding actogram-style plots, and drawn a blue line through the trough of body temperature that occurred (without explanation) in the middle of mealtime in waveform C, and through the peak in temperature that occurred in the middle of mealtime in waveform D. Clearly, the peak in temperature in D is not reflected by the density of the same data in B. In addition, the temperature curve in both waveforms is a mirror image on either side of the blue line. Therefore, in the actogram-style plots of the same data, the dark sections (indicating higher temperature) should also be symmetrical on either side of the blue line. They are not. In actogram-style plot B, high temperature is indicated during the first 1–2 h of mealtime, yet the corresponding waveform shows a lower temperature (likely below the daily mean) during that time.

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