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Figure 1 | Journal of Circadian Rhythms

Figure 1

From: Magel2, a Prader-Willi syndrome candidate gene, modulates the activities of circadian rhythm proteins in cultured cells

Figure 1

Magel2 represses Clock:Bmal1-mediated transcription at an E-box promoter. Dual luciferase transcription assays following transfection of NIH3T3 cells with the mPer2:luc reporter plasmid and a renilla internal control plasmid, together with combinations of expression plasmids variously encoding epitope-tagged Clock, Bmal1, Magel2, Cry1, and Per2 proteins. Data are normalized to the baseline activity of the normalized mPer2:luc reporter. A * indicates a significant difference from the baseline mPer2:luc activity, and a # indicates a significant repression compared to Bmal1:Clock-mediated activation (p < 0.05). These results represent one of three experiments completed in triplicate. Error bars represent the standard error of the mean.

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Journal of Circadian Rhythms

ISSN: 1740-3391